Forsøksdyr: Characterization of Mettl4 in mice

Godkjenningsdato 15.05.2020

Godkjenningsperiode 01.06.2020-31.05.2024

I) The purpose of the experiment:
Study the role of Mettl4 in vivo.
II) The expected adverse effects on the animals:
These animals are characterized because they have a deleted exon in the Mettl4 gene. Neither the knock-out (KO) animals nor the heterozygous ones have any difficulties compared to the wild-type mice.
III) The expected scientific benefits or benefits for society:
The more we research on methyl-6-adenosine mRNA modifications the more we can relate it to important processes like development or cancer. Mettl4 is supposed to be a part of the m6A network and we would like to unravel the function at in vivo level of this protein.
IV) The number of animals and species:
We would like to use 661 mice, Mus musculus, strain C56BL/6J-Mettl4<em1Aklu> (including wild-type, heterozygous for Mettl4 and KO).
V) How will the requirements for 3R be accomplished by the experiment/project:
For all of our mice projects we replace the animals with cell lines derived from them as much as we can, we will develop neural stem cells whenever possible for our purpose. We have thoroughly calculated the amount of animals that we need to obtain significant results, and the types of breedings that we need to reduce the amount of animals as much as possible. Also, our group has a lot of experience in animal projects, not only with constitutive knock out lines (like this one) but also with conditional and reporter lines. This experience helps us refine all the mouse handling and procedures.